EXTH-24. FILAMENTOUS PHAGE AS A NANOTHERANOSTIC FOR THE DETECTION, IMAGING, AND TREATMENT OF GLIOMAS

Abstract Current standard of care treatment for gliomas includes maximal safe surgical resection followed by concurrent chemoradiation, however, the diffuse infiltrative nature present certain challenges neurosurgeons to clearly visualize tumor tissue under conventional white light during microsurgical resection. Fluorescence-guided techniques have been recently introduced help surgeons better operating microscope. Fluorescent dyes conjugated nanoscale materials that are functionalized with glioma-targeting peptide sequences, such as 39 amino acid chlorotoxin (CTX) sequence, demonstrated clinical efficacy in recognizing microscopic cells surgeries. M13 bacteriophage (phage) versatile, genetically tunable nanocarriers adapted use theranostic platforms. Applying p3 capsid CTX fusion a unique “inho” circular single-stranded DNA (cssDNA) gene packaging system, we produced miniature inho (CTX-inho phage particles minimum length 50 nm can target intracranial orthotopic patient-derived glioblastoma tumors brains mice. Systemically-administered indocyanine green-conjugated CTX-inho accumulated brain tumors, facilitating short-wave infrared detection. Furthermore, show our carry cssDNA transcriptionally active when delivered GBM22 glioma vitro. The ability modulate display, surface loading, length, and content makes recombinant particle an ideal nanotheranostic platform neuro-oncological applications.